All lanes : Anti-Mitofusin 2 antibody (ab50843) at 0.25 µg/mlLane 1 : Rat brain mitochondriaLane 2 : Mouse brain mitochondriaSecondaryGoat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate.
ab50843 staining Mitofusin 2 in murine cardiac myocyte cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde, permeabilized using 1% Triton X-100, blocked with 10% serum for 1 hour at room temperature, then incubated with ab50843 at a 1/200 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.See Abreview