Anti-Mkl1 antibody
| Name | Anti-Mkl1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab49311 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IP WB ELISA |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Horse, Bovine, Cat, Dog, Pig |
| Antigen | Synthetic peptide: QPLSQPGFPA PGPPAQMDLE HPPQPPFATP TSLLKKEPPG YEETVTQQPk, corresponding to amino acids 721-770 of Mouse Mkl1 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | MKL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Mkl1 antibody (ab49311) at 1.25 µg/ml + NIH3T3 lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab49311 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49311, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.