MKL1

Gene Symbol MKL1
Entrez Gene 57591
Alt Symbol BSAC, MAL, MRTF-A
Species Human
Gene Type protein-coding
Description megakaryoblastic leukemia (translocation) 1
Other Description MKL/myocardin-like protein 1|Mkl1 variant 1 Mkl1_S|basic, SAP and coiled-coil domain|megakaryoblastic leukemia 1 protein|megakaryocytic acute leukemia protein|myocardin-related transcription factor A
Swissprots Q96SC6 Q96SC5 Q8TCL1 Q969V6 Q9P2B0
Accessions EAW60382 EAW60383 EAW60384 Q969V6 AB037859 BAA92676 AF368061 AAK54721 AF448806 AJ297257 CAC38826 AJ297258 CAC38827 AL713710 CAD28507 BC021586 BC064620 BC114364 AAI14365 BC115039 AAI15040 BC139730 BC141653 AAI41654 BC141655 AAI41656 BG716386 CR456522 CAG30408 CU013122 CAK54553 CU013410 CAK54852 HG764171 CDJ79779 HY058235 HY123688 XM_005261691 XP_005261748 XM_005261692 XP_005261749 XM_005261694 XP_005261751 XM_011530283 XP_011528585 XM_011530284 XP_011528586 XM_011530285 XP_011528587 XM_011530286 XP_011528588 XM_011530287 XP_011528589 NM_001282660 NP_001269589 NM_001282661 NP_001269590 NM_001282662 NP_001269591 NM_020831 NP_065882
Function Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation (By similarity). {ECO:0000250}.
Subcellular Location Nucleus {ECO:0000269|PubMed:19350017, ECO:0000269|PubMed:24440334}. Cytoplasm {ECO:0000250}. Note=Binding to globular actin is required to maintain cytoplasmic localization. Nuclear localization is regulated by MICAL2, which mediates depolymerization of nuclear globular actin, promoting retention of MKL1 in the nucleus and subsequent formation of an active complex with SRF (PubMed:24440334). {ECO:0000269|PubMed:24440334}.
Tissue Specificity Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.

MKL1/MRTF-A Antibody - 14760 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 and HeLa cells using MKL1/MRTF-A Antibody

MRTF-A (G-7) - sc-398675 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MRTF-A (G-7): sc-398675. Western blot analysis of MRTF-A expression in HeLa (A) and A549 (B) nuclear extracts.

MRTF-A (G-8) - sc-390324 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MRTF-A (G-8): sc-390324. Western blot analysis of MRTF-A expression in NIH/3T3 (A) and A549 (B) nuclear extracts and HeLa (C) and NIH/3T3 (D) whole cell lysates.

MRTF-A (C-19) - sc-21558 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MRTF-A (C-19): sc-21558. Western blot analysis of MRTF-A expression in HeLa nuclear extract.

MRTF-A (H-140) - sc-32909 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MRTF-A (H-140): sc-32909. Western blot analysis of MRTF-A expression in HeLa (A) and A549 (B) nuclear extracts.

Anti-Mkl1 antibody - ab49311 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Mkl1 antibody (ab49311) at 1.25 µg/ml + NIH3T3 lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Mkl1 antibody - ab115319 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab115319 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115319 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Mkl1 antibody - ab138745 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Mkl1 antibody (ab138745) at 1/100 dilution + CEM cell lysate at 35 µg

Anti-Mkl1 antibody - ab113264 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed, paraffin-embedded section of Human breast carcinoma. Antibody: ab113264 used at a dilution of 1/250. Detection: DAB staining.

Anti-BSAC / MKL1 Antibody (aa497-525, AP) - LS-C223276 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BSAC / MKL1 Antibody (aa497-525, APC) - LS-C223277 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BSAC / MKL1 Antibody (aa497-525, Biotin) - LS-C223278 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BSAC / MKL1 Antibody (aa497-525, Azide-free, HRP) - LS-C223280 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BSAC / MKL1 Antibody (aa497-525, FITC) - LS-C223279 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BSAC / MKL1 Antibody (aa721-770) - LS-C31461 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
NIH 3T3 Cell Lysate

Anti-BSAC / MKL1 Antibody (aa750-800) - LS-C288956 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-BSAC / MKL1 Antibody (aa497-525, PE) - LS-C223281 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BSAC / MKL1 Antibody (aa10-59) - LS-C119199 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HUVEC/HeLa cells, using MKL1 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-BSAC / MKL1 Antibody (aa496-525) - LS-C323015 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-BSAC / MKL1 Antibody (His11) - LS-C176943 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of MRTF-A (H11) pAb in extracts from HUVEC cells.

Anti-BSAC / MKL1 Antibody (aa700-750) IHC-plus™ - LS-B11058 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-BSAC / MKL1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11058 dilution 1:100.

Anti-BSAC / MKL1 Antibody (aa497-525) - LS-C158145 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
MKL1 Antibody western blot of CEM cell line lysates (35 ug/lane). The MKL1 antibody detected the MKL1 protein (arrow).

Anti-BSAC / MKL1 Antibody (aa165-397, clone 4F6) - LS-C339473 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4F6
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKL1.

Anti-BSAC / MKL1 Antibody (aa165-397, clone 4D10) - LS-C339474 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4D10
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKL1.

Anti-BSAC / MKL1 Antibody (aa165-397, clone 2B3) - LS-C339492 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKL1.
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