Anti-MKLP1 antibody

• Supplier: Abcam
• Catalog: ab9259
• Prices: $379.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IP WB
• Species Reactivities: Human, Chimpanzee, Ape
• Antigen: Synthetic peptide (Human) conjugated to KLH - which represented a portion within the first 100 amino acids of the human Mitotic Kinesin-Like Protein-1 (GenBank PID 400264)
• Description: Rabbit Polyclonal
• Gene: KIF23
• Conjugate: Unconjugated

Product images

ICC/IF image of ab9259 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Cardiomyocyte proliferation contributes to heart growth in young humans. - Cardiomyocyte proliferation contributes to heart growth in young humans.

Mollova M, Bersell K, Walsh S, Savla J, Das LT, Park SY, Silberstein LE, Dos Remedios CG, Graham D, Colan S, Kuhn B. Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1446-51. doi:

Megakaryocyte endomitosis is a failure of late cytokinesis related to defects in - Megakaryocyte endomitosis is a failure of late cytokinesis related to defects in

Lordier L, Jalil A, Aurade F, Larbret F, Larghero J, Debili N, Vainchenker W, Chang Y. Blood. 2008 Oct 15;112(8):3164-74.