Anti-MKLP1 antibody [EPR10879]

• Supplier: Abcam
• Catalog: ab174304
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR10879
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MKLP1 aa 800-900 (Cysteine residue)
• Description: Rabbit Monoclonal
• Gene: KIF23
• Conjugate: Unconjugated

Product images

Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/5000 dilution (purified) + RAW264.7 cell lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/1000 dilution (purified) + Rat lung tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/20000 dilution (purified) + HeLa cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/5000 dilution (purified)Lane 1 : HEK293 cell lysateLane 2 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunofluorescence staining of HeLa cells with purified ab174304 at a working dilution of 1 in 400, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab174304 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

All lanes : Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/1000 dilution (unpurified)Lane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : A549 cell lysateLysates/proteins at 10 µg per lane.

Immunofluorescent analysis of A547 cells labeling MKLP1 with unpurified ab174304 at 1/100.