Anti-MLC1 antibody
| Name | Anti-MLC1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab94730 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat, Horse, Guinea Pig, Bovine, Dog |
| Antigen | Synthetic peptide corresponding to a region within internal amino acids 180 - 229 (DSANILDEVP FPARVLKSYS VVEVIAGISA VLGGIIALNV DDSVSGPHLS) of Human MLC1 (NP_055981) |
| Description | Rabbit Polyclonal |
| Gene | MLC1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab94730 staining MLC1 in primary Rat cortical astrocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde. After 1 hour of incubation with blocking solution (5% BSA in PBS), cells were incubated for 1 hour at room temperature with a NBD phallacidin high-affinity F-actin probe (1/30) to stain actin filaments and visualize the whole cell body, used in combination with ab94730 (1/50) diluted in PBS+0.2% Triton X100.To amplify MLC1 staining, a biotinilated secondary antibody (4.3 µg/ml, Biotin-SP-AffiniPure goat anti-rabbit IgG H+L) followed by incubation with 2 µg/ml streptavidin-TRITC was used.
Anti-MLC1 antibody (ab94730) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal spleen lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
Anti-MLC1 antibody (ab94730) at 1/500 dilution + Mouse brain tissue lysate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.