Anti-MLKL (phospho S358) antibody [EPR9514]

• Supplier: Abcam
• Catalog: ab187091
• Prices: $378.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9514
• Applications: WB IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MLKL aa 350-450 (phospho S358)
• Description: Rabbit Monoclonal
• Gene: MLKL
• Conjugate: Unconjugated

Product images

All lanes : Anti-MLKL (phospho S358) antibody [EPR9514] (ab187091) at 1/2000 dilutionLane 1 : Untreated HT-29 lysateLane 2 : HT-29 cell lysate treated with TNF alpha+ Smac mimetic+ z-VADLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

ab187091 at 1:250 staining MLKL (phospho S358) in Human skin tissue by immunohistochemistry (FFPE). Antigen retrival required on FFPE tissue: HIER using 10mM Citrate buffer pH 6.0, see recommended HIER protocolFor additional IHC guideline, please see IHC resource page

ab187091 at 1:250 staining MLKL (phospho S358) in Human melanoma tissue by immunohistochemistry (FFPE). Antigen retrival required on FFPE tissue:  HIER using 10mM Citrate buffer pH 6.0, see recommended HIER protocol For additional IHC guideline, please see IHC resource page

Product References

Mixed lineage kinase domain-like protein MLKL causes necrotic membrane disruption - Mixed lineage kinase domain-like protein MLKL causes necrotic membrane disruption

Wang H, Sun L, Su L, Rizo J, Liu L, Wang LF, Wang FS, Wang X. Mol Cell. 2014 Apr 10;54(1):133-46.

Carbamoylating activity associated with the activation of the antitumor agent - Carbamoylating activity associated with the activation of the antitumor agent

Ji W, Yang M, Praggastis A, Li Y, Zhou HJ, He Y, Ghazvinian R, Cincotta DJ, Rice KP, Min W. PLoS One. 2014 Jul 28;9(7):e103224.

Mixed lineage kinase domain-like protein mediates necrosis signaling downstream - Mixed lineage kinase domain-like protein mediates necrosis signaling downstream

Sun L, Wang H, Wang Z, He S, Chen S, Liao D, Wang L, Yan J, Liu W, Lei X, Wang X. Cell. 2012 Jan 20;148(1-2):213-27.