MLKL

Gene Symbol MLKL
Entrez Gene 197259
Alt Symbol hMLKL
Species Human
Gene Type protein-coding
Description mixed lineage kinase domain-like
Other Description mixed lineage kinase domain-like protein
Swissprots Q8NB16 A6NCE4 Q8N6V0
Accessions CAH25906 EAW95679 Q8NB16 AK091708 BAC03728 AK093013 AK122998 AL833362 CAI46144 BC028141 AAH28141 BQ575071 XM_005255834 XP_005255891 XM_011522934 XP_011521236 XM_011522935 XP_011521237 XM_011522936 XP_011521238 XR_243397 XR_429715 XR_429716 XR_933232 XR_933233 NM_001142497 NP_001135969 NM_152649 NP_689862
Function Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. {ECO:0000269|PubMed:22265413, ECO:0000269|PubMed:22265414, ECO:0000269|PubMed:22421439, ECO:0000269|PubMed:24316671}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:24316671}. Cell membrane {ECO:0000269|PubMed:24316671}. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.
Top Pathways TNF signaling pathway

Phospho-MLKL (Thr357/Ser358) Antibody - 14516 from Cell Signaling Technology

Prices $279.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT-29 cells, untreated (-) or pre-treated with Z-VAD (20 μM, 30 min) followed by overnight treatment with Cycloheximide #2112 (CHX, 10 μg/ml) and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml; +), using Phospho-MLKL (Thr357/Ser358) Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

MLKL (Y-14) - sc-165025 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MLKL (Q-15) - sc-165024 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-MLKL (phospho S358) antibody [EPR9514] - ab187091 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9514
All lanes : Anti-MLKL (phospho S358) antibody [EPR9514] (ab187091) at 1/2000 dilutionLane 1 : Untreated HT-29 lysateLane 2 : HT-29 cell lysate treated with TNF alpha+ Smac mimetic+ z-VADLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-MLKL antibody - ab118348 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HEK293 cells stained with ab118348 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118348, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-MLKL antibody - ab189612 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MLKL antibody (ab189612) at 1/1000 dilutionLane 1 : HeLa total cell extractLane 2 : Jurkat total cell extract

Anti-MLKL antibody - ab67942 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-MLKL antibody (ab67942) at 1/500 dilutionLane 1 : MLKL transfected 293T cell lysateLane 2 : non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Anti-MLKL antibody - ab183770 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MLKL antibody (ab183770) at 1/1000 dilutionLane 1 : THP-1 whole cell lysateLane 2 : NCI-H929 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-MLKL antibody [EPR17514] - ab184718 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17514
All lanes : Anti-MLKL antibody [EPR17514] (ab184718) at 1/20000 dilutionLane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysateLane 2 : HT-29 (Human colorectal adenocarcinoma cells) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-MLKL antibody - N-terminal - ab194699 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-MLKL antibody - N-terminal (ab194699) at 1/500 dilution + THP1 cell lysate

Anti-MLKL Antibody (aa443-472, Biotin) - LS-C263236 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MLKL Antibody (aa443-472, AP) - LS-C263232 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MLKL Antibody (N-Terminus, Biotin) - LS-C279617 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-MLKL Antibody (aa443-472, Azide-free, HRP) - LS-C263238 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MLKL Antibody (aa443-472, APC) - LS-C263233 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MLKL Antibody (N-Terminus, AP) - LS-C279615 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-MLKL Antibody (aa443-472, FITC) - LS-C263237 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MLKL Antibody (aa443-472, PE) - LS-C263239 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MLKL Antibody (N-Terminus, APC) - LS-C279616 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-MLKL Antibody (N-Terminus, FITC) - LS-C279618 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-MLKL Antibody (N-Terminus, PE) - LS-C279620 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-MLKL Antibody (N-Terminus, Azide-free, HRP) - LS-C279619 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-MLKL Antibody (aa107-156) - LS-C111469 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MLKL antibody LS-C111469 Western blot of Fetal Kidney lysate.

Anti-MLKL Antibody - LS-C309015 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-MLKL Antibody (aa443-472) - LS-C323026 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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