Anti-MMP12 antibody
| Name | Anti-MMP12 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab38935 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide based on the hinge region of Human MMP12 |
| Description | Rabbit Polyclonal |
| Gene | MMP12 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-MMP12 antibody (ab38935) + Cell media from human metastatic melanoma (treated with TNF alpha)Observed band size : 45,54 kDa (why is the actual band size different from the predicted?)
ICC/IF image of ab38935 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38935, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.