MMP12

Gene Symbol MMP12
Entrez Gene 4321
Alt Symbol HME, ME, MME, MMP-12
Species Human
Gene Type protein-coding
Description matrix metallopeptidase 12
Other Description macrophage metalloelastase|matrix metallopeptidase 12 (macrophage elastase)|matrix metalloproteinase 12 (macrophage elastase)
Swissprots B7ZLF6 B2R9X8 Q2M1L9 P39900
Accessions AAB06314 AAB36943 AAW29944 EAW67033 P39900 AI343504 AI352562 AK313959 BAG36675 AW172994 BC112301 AAI12302 BC143773 AAI43774 BE440047 BE813419 HQ258263 ADR83017 L23808 AAA58658 NM_002426 NP_002417
Function May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Subcellular Location Secreted, extracellular space, extracellular matrix {ECO:0000305}.
Tissue Specificity Found in alveolar macrophages but not in peripheral blood monocytes.

MMP-12 (E-16) - sc-31805 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MMP-12 (K-15) - sc-12361 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MMP-12 (K-15): sc-12361. Western blot analysis of human recombinant MMP-12. Note catalytic domain (~25 kDa) generated by autoproteolysis.

MMP-12 (S-12) - sc-31807 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MMP-12 (W-15) - sc-31809 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MMP-12 (H-300) - sc-30072 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MMP-12 (H-300): sc-30072. Immunofluorescence staining of normal mouse intestine frozen section showing extracellular matrix staining.

MMP-12 (MM0025-7G20) - sc-101449 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

Anti-MMP12 antibody - ab137444 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab137444 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab137444 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MMP12 antibody - ab137443 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MMP12 antibody (ab137443) at 1/1000 dilution + 293T whole cell lysate at 30 µg

Anti-MMP12 antibody - ab38935 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-MMP12 antibody (ab38935) + Cell media from human metastatic melanoma (treated with TNF alpha)Observed band size : 45,54 kDa (why is the actual band size different from the predicted?)

Anti-MMP12 antibody - ab113514 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MMP12 antibody (ab113514) at 1 µg/mlLane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MMP12 antibody - ab26832 from Abcam

Host Chicken
Clonality Polyclonal
Anti-MMP12 antibody (ab26832) at 1/5000 dilution + human MMP12 protein 400ng (1-33amino acids)Secondarygoat-anti-IgY/Fc-HRPdeveloped using the ECL technique

Anti-MMP12 antibody - ab128030 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab128030, at a concentration of 5 µg/ml, staining MMP12 in formalin fixed, paraffin embedded Human lung macrophages by Immunohistochemistry.

Anti-MMP12 antibody - ab38911 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MMP12 antibody (ab38911)Lane 1 : Cell media from human lung fetal fibroblasts (treated with IL1 beta)Lane 2 : Cell media from human chondrosarcoma (treated with IL1 beta)

Anti-MMP12 antibody - ab99473 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-MMP12 antibody (ab99473) at 0.5 µg/ml + Human breast cancer lysate at 35 µgdeveloped using the ECL technique

Anti-MMP12 antibody - ab172042 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-MMP12 antibody (ab172042) at 1 µg/mlLane 1 : MMP12 transfected 293T cell lysateLane 2 : Non-transfected cell lysateLysates/proteins at 15 µl per lane.

Anti-MMP12 antibody [EP1261Y] - ab52897 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EP1261Y

Anti-MMP12 antibody [EP1261Y] (Alexa Fluor® 488) - ab200409 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1261Y
ab200409 staining MMP12 in A549 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200409 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-MMP12 antibody [EP1261Y] (Alexa Fluor® 647) - ab200410 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1261Y
ab200410 staining MMP12 in A549 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200410 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-MMP12 antibody [MM0025-7G20] - ab56305 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MM0025-7G20
ab56305 at 1/300 dilution, staining MMP12 in human placenta tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

Anti-MMP12 antibody - Aminoterminal end - ab38908 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-MMP12 antibody - Aminoterminal end (ab38908) at 1/1000 dilution + Cell media from human chondrosarcoma (treated with IL1)

Anti-MMP12 antibody - Carboxyterminal end - ab66157 from Abcam

Prices $401.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Ab66157 staining human breast carcinoma. Staining is localized to the nuclear membrane and cytoplasm.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-MMP12 antibody - Carboxyterminal end - ab38910 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-MMP12 antibody - Carboxyterminal end (ab38910) + Cell media from human embryonal rhabdomyosarcoma (treated with TNF alpha)

Anti-MMP12 antibody - Carrier Free - ab128974 from Abcam

Prices $3,178.00
Sizes 1 mg
Host Rabbit
Clonality Polyclonal

Anti-MMP12 Antibody (aa406-420) - LS-C3113 from LifeSpan Bioscience

Prices $285.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MMP12 Antibody - LS-C48498 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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