Anti-MMP12 antibody [EP1261Y]
| Name | Anti-MMP12 antibody [EP1261Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52897 |
| Prices | $99.00 |
| Sizes | 10 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1261Y |
| Applications | WB IP FC IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues on the C-terminus of human MMP12 |
| Description | Rabbit Monoclonal |
| Gene | MMP12 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab52897 (1:100) staining human MMP12 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.Ab52897 (1:100) staining human MMP12 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Immunofluorescent staining of HeLa cells using ab52897 (1:100).
Overlay histogram showing A549 cells stained with ab52897 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52897, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG, H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
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