Anti-MMP12 antibody - Carboxyterminal end
| Name | Anti-MMP12 antibody - Carboxyterminal end |
|---|---|
| Supplier | Abcam |
| Catalog | ab66157 |
| Prices | $401.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB IP ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Rabbit |
| Antigen | Synthetic peptide corresponding to the C-terminus of human MMP12 |
| Description | Rabbit Polyclonal |
| Gene | MMP12 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab66157 staining human breast carcinoma. Staining is localized to the nuclear membrane and cytoplasm.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
All lanes : Anti-MMP12 antibody - Carboxyterminal end (ab66157) at 1 µg/mlLane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 2 : WI38 (Human lung fibroblast cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab66157 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66157, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12 - Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12
Krarup PM, Eld M, Heinemeier K, Jorgensen LN, Hansen MB, Agren MS. Int J Colorectal Dis. 2013 Aug;28(8):1151-9.
Increased serum levels and tissue expression of matrix metalloproteinase-12 in - Increased serum levels and tissue expression of matrix metalloproteinase-12 in
Manetti M, Guiducci S, Romano E, Bellando-Randone S, Conforti ML, Ibba-Manneschi L, Matucci-Cerinic M. Ann Rheum Dis. 2012 Jun;71(6):1064-72.
Effects of short-term cigarette smoke exposure on Fischer 344 rats and on - Effects of short-term cigarette smoke exposure on Fischer 344 rats and on
Carter CA, Misra M. Toxicol Pathol. 2010 Apr;38(3):402-15.