Anti-MMP13 antibody [VIIIA2]
| Name | Anti-MMP13 antibody [VIIIA2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab3208 |
| Prices | $379.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | VIIIA2 |
| Applications | IHC-P IP IHC-F ICC/IF ICC/IF |
| Species Reactivities | Rat, Human, Mouse |
| Antigen | Human recombinant collagenase 3 protein |
| Description | Mouse Monoclonal |
| Gene | MMP13 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab3208 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3208, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab3208 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3208, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Lipid-soluble cigarette smoking particles induce expression of inflammatory and - Lipid-soluble cigarette smoking particles induce expression of inflammatory and
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Transcriptional regulation of inflammatory and extracellular matrix-regulating - Transcriptional regulation of inflammatory and extracellular matrix-regulating
Vikman P, Ansar S, Edvinsson L. J Neurosurg. 2007 Nov;107(5):1015-22.