All lanes : Anti-MMP15 antibody - N-terminal (ab135562) at 1/100 dilutionLane 1 : Mouse brain tissue lysateLane 2 : HL60 cells lysateLysates/proteins at 12.5 µg per lane.
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human colon carcinoma tissue labelling MMP15 with ab135562 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of WiDr cells labelling MMP15 with ab135562 at 1/10 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.