Anti-MMP2 antibody [4D3]
| Name | Anti-MMP2 antibody [4D3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab2462 |
| Prices | $390.00 |
| Sizes | 1 ml |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 4D3 |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Rabbit, Human, Mouse, Rat |
| Antigen | Synthetic peptide corresponding to Human MMP2 aa 557-569 (internal sequence) |
| Description | Mouse Monoclonal |
| Gene | MMP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
400 ng samples of recombinant human pro-enzyme (lane 1 = MMP-1; lane 2 = MMP-3; lane 3 = MMP-2; lane 4 = MMP-9) were subjected to SDS-PAGE, transferred to PVDF membrane, and detected by immunoblotting with ab2462 specific for MMP-2.
![Anti-MMP2 antibody [4D3] (ab2462) at 1/5000 dilution + Rabbit aorta tissue lysate at 40 µgSecondaryHRP-conjugate Sheep anti-mouse IgG polyclonaldeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 72 kDa (why is the actual band size different from the predicted?)Exposure time : 30 secondsThis image is courtesy of an anonymous AbreviewSee Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/23462_ab2462-197048-ab2462wb.jpg)
Anti-MMP2 antibody [4D3] (ab2462) at 1/5000 dilution + Rabbit aorta tissue lysate at 40 µgSecondaryHRP-conjugate Sheep anti-mouse IgG polyclonaldeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 72 kDa (why is the actual band size different from the predicted?)Exposure time : 30 secondsThis image is courtesy of an anonymous AbreviewSee Abreview
ab2462 staining MMP2 in HT-1080 cells by Immunocytochemistry/ Immunofluorescence.Cells were grown on glass coverslips, treated with PMA and then fixed with 4% (w/v) paraformaldehyde in phosphate buffered saline (PBS). Samples were then permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% (w/v) skimmed milk powder in PBS before staining with ab2462 and the appropriate secondary antibody, followed by washing and mounting.Scale bar: 10 µm.
IHC of human oesophageal adenocarcinoma labelling MMP2 with ab2462 (undiluted).
Product References
Matrix metalloproteinases and their inhibitors and inducer in gestational - Matrix metalloproteinases and their inhibitors and inducer in gestational
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Differential gene expression in normal esophagus and Barrett's esophagus. - Differential gene expression in normal esophagus and Barrett's esophagus.
Wang J, Qin R, Ma Y, Wu H, Peters H, Tyska M, Shaheen NJ, Chen X. J Gastroenterol. 2009;44(9):897-911.
Matrix metalloproteinases and their inhibitors in gastric cancer. - Matrix metalloproteinases and their inhibitors in gastric cancer.
Murray GI, Duncan ME, Arbuckle E, Melvin WT, Fothergill JE. Gut. 1998 Dec;43(6):791-7.
Matrix metalloproteinase-1 is associated with poor prognosis in oesophageal - Matrix metalloproteinase-1 is associated with poor prognosis in oesophageal
Murray GI, Duncan ME, O'Neil P, McKay JA, Melvin WT, Fothergill JE. J Pathol. 1998 Jul;185(3):256-61.