Anti-MMP2 antibody [6E3F8]

• Supplier: Abcam
• Catalog: ab86607
• Prices: $388.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone: 6E3F8
• Applications: IHC-F WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment derived from within residues 380 - 655 of Human MMP2
• Description: Mouse Monoclonal
• Gene: MMP2
• Conjugate: Unconjugated

Product images

All lanes : Anti-MMP2 antibody [6E3F8] (ab86607) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 2 : Liver (Mouse) Tissue Lysate Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 4 : Spleen (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

IHC image of ab86607 staining in Human Breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86607, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-FoFr image of MMP2 staining on rat hippocampal sections using ab86607 (1:300). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. hippocampus was then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.See Abreview

All lanes : Anti-MMP2 antibody [6E3F8] (ab86607) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : A375 (Human melanoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Product References

Increased expression of prostasin contributes to early-onset severe preeclampsia - Increased expression of prostasin contributes to early-onset severe preeclampsia

Yang Y, Zhang J, Gong Y, Liu X, Bai Y, Xu W, Zhou R. J Perinatol. 2015 Jan;35(1):16-22.

MiR200-upregulated Vasohibin 2 promotes the malignant transformation of tumors by - MiR200-upregulated Vasohibin 2 promotes the malignant transformation of tumors by

Xue X, Zhang Y, Zhi Q, Tu M, Xu Y, Sun J, Wei J, Lu Z, Miao Y, Gao W. Cell Commun Signal. 2014 Oct 1;12(1):62.

Topical treatment with the opioid antagonist naltrexone accelerates the - Topical treatment with the opioid antagonist naltrexone accelerates the

Immonen JA, Zagon IS, Lewis GS, McLaughlin PJ. Exp Biol Med (Maywood). 2013 Oct;238(10):1127-35.