Anti-MMP2 antibody [CA-4001 / CA719E3C]
| Name | Anti-MMP2 antibody [CA-4001 / CA719E3C] |
|---|---|
| Supplier | Abcam |
| Catalog | ab3158 |
| Prices | $391.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | CA-4001 / CA719E3C |
| Applications | FC IHC-P IP WB |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthetic peptide: APSPIIKFPGD-VAPKTDK , corresponding to amino acids 30-47 of MMP2 |
| Description | Mouse Monoclonal |
| Gene | MMP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-MMP2 antibody [CA-4001 / CA719E3C] (ab3158) at 1/50 dilution + human fetal lung cell-1 lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/23471_MMP2-Primary-antibodies-ab3158-1.jpg)
Anti-MMP2 antibody [CA-4001 / CA719E3C] (ab3158) at 1/50 dilution + human fetal lung cell-1 lysate
IHC image of ab3158 staining in Hu placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3158, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemical analysis of rat aorta tissue, staining MMP2 with ab3158 at 1/100 dilution. Staining was detected using DAB.
![Overlay histogram showing MCF7 cells stained with ab3158 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3158, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/23474_MMP2-Primary-antibodies-ab3158-3.jpg)
Overlay histogram showing MCF7 cells stained with ab3158 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3158, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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