Anti-MNK1 antibody (ab89223) at 5 µg/ml + immunogen at 0.2 µgdeveloped using the ECL technique
Anti-MNK1 antibody (ab89223) at 5 µg/ml + Raw 264.7 cell lysate at 50 µgdeveloped using the ECL technique
ab89223, at 3 µg/ml, staining MNK1 in formalin fixed, paraffin embedded human small intestine tissue by Immunoperoxidase/Immunohistochemistry.
ab89223, at 10 µg/ml, staining MNK1 in paraformaldehyde fixed, permeabilised HeLa cell by Immunofluorescence.
![Overlay histogram showing HeLa cells stained with ab89223 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89223, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/23780_MNK1-Primary-antibodies-ab89223-5.jpg)
Overlay histogram showing HeLa cells stained with ab89223 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89223, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.