![Overlay histogram showing HepG2 cells stained with ab119777 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119777, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/23946_ab119777-9-ab119777FC.jpg)
Overlay histogram showing HepG2 cells stained with ab119777 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119777, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-Monoacylglycerol Lipase antibody [1B1] (ab119777) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Monoacylglycerol Lipase cDNALysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/23947_Monoacylglycerol-Lipase-Primary-antibodies-ab119777-1.jpg)
All lanes : Anti-Monoacylglycerol Lipase antibody [1B1] (ab119777) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Monoacylglycerol Lipase cDNALysates/proteins at 5 µg per lane.
ab119777, at 1/100 dilution, staining Monoacylglycerol Lipase in COS7 cells transiently transfected with pCMV6-ENTRY Monoacylglycerol Lipase cDNA by Immunofluorescence
ab119777, at 1/150 dilution, staining Monoacylglycerol Lipase in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry
ab119777, at 1/150 dilution, staining Monoacylglycerol Lipase in paraffin-embedded Human kidney carcinoma tissue by Immunohistochemistry.
ab119777, at 1/150 dilution, staining Monoacylglycerol Lipase in paraffin-embedded Human lymphoma tissue by Immunohistochemistry.
ab119777, at 1/150 dilution, staining Monoacylglycerol Lipase in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
ab119777, at 1/150 dilution, staining Monoacylglycerol Lipase in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
ab119777, at 1/150 dilution, staining Monoacylglycerol Lipase in paraffin-embedded Human tonsil tissue by Immunohistochemistry.