All lanes : Anti-Mps1 antibody (ab24227)Lane 1 : HeLa cell lyste at 15µg and ab at 0.033ug/mlLane 2 : HeLa cell lyste at 50ug and ab at 0.033ug/mlLane 3 : HeLa cell lyste at 50ug and ab at 0.33ug/mlLane 4 : HeLa cell lyste at 50ug and ab at 0.33ug/mlLane 5 : HeLa cell lyste at 2mg and ab at 1ug/2mg lysateLane 6 : HeLa cell lyste at 2mg and ab at 1ug/2mg lysateLane 7 : HeLa cell lyste at 2mg and ab at 1ug/2mg lysatedeveloped using the ECL technique
ICC/IF image of ab24227 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24227, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.