Anti-MRP3 antibody [M3II-9]

• Supplier: Abcam
• Catalog: ab3375
• Prices: $388.00
• Sizes: 250 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: M3II-9
• Applications: FC ICC/IF IHC-F WB IHC-P
• Species Reactivities: Mouse, Human
• Antigen: Fusion protein: SEMGPYPALL QRNGSFANFL CNYAPDEDQG HLEDSWTALE GAEDKEALLI EDTLSNHTD LTDNDPVTYV VQKQFMRQLS ALSSDGEGQG RPVPRRHLGPS EKVQVTEAKA DGALTQEEKA , corresponding to amino acids 830-949 of Human MRP3
• Description: Mouse Monoclonal
• Gene: ABCC3
• Conjugate: Unconjugated

Product images

developed using the ECL techniquePerformed under reducing conditions.Exposure time : 20 minutes HepG2 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of clotrimazole (ab120744) in DMSO. Increased expression of MRP3 (ab3375) correlates with an increase in clotrimazole concentration, as described in literature.Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3375 at 1/50 dilution and ab9484 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.

Product References

Molecular mechanism of altered ezetimibe disposition in nonalcoholic - Molecular mechanism of altered ezetimibe disposition in nonalcoholic

Hardwick RN, Fisher CD, Street SM, Canet MJ, Cherrington NJ. Drug Metab Dispos. 2012 Mar;40(3):450-60.

Variations in ATP-binding cassette transporter regulation during the progression - Variations in ATP-binding cassette transporter regulation during the progression

Hardwick RN, Fisher CD, Canet MJ, Scheffer GL, Cherrington NJ. Drug Metab Dispos. 2011 Dec;39(12):2395-402.