Anti-MRP4 antibody
| Name | Anti-MRP4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56675 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | ICC/IF ICC/IF WB FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 1-110 of Human MRP4 |
| Description | Mouse Monoclonal |
| Gene | ABCC4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab56675 stained HepG2 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56675, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing PC3 cells stained with ab56675 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56675, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/24480_MRP4-Primary-antibodies-ab56675-2.jpg)
Overlay histogram showing PC3 cells stained with ab56675 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56675, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Cellular uptake and antiproliferative effects of 11-oxo-eicosatetraenoic acid. - Cellular uptake and antiproliferative effects of 11-oxo-eicosatetraenoic acid.
Snyder NW, Revello SD, Liu X, Zhang S, Blair IA. J Lipid Res. 2013 Nov;54(11):3070-7.
The multidrug resistance-associated protein 4 (MRP4) appears as a functional - The multidrug resistance-associated protein 4 (MRP4) appears as a functional
Lacroix-Pepin N, Danyod G, Krishnaswamy N, Mondal S, Rong PM, Chapdelaine P, Fortier MA. Endocrinology. 2011 Dec;152(12):4993-5004.
MRP4 knockdown enhances migration, suppresses apoptosis, and produces aggregated - MRP4 knockdown enhances migration, suppresses apoptosis, and produces aggregated
Tagami M, Kusuhara S, Imai H, Uemura A, Honda S, Tsukahara Y, Negi A. Biochem Biophys Res Commun. 2010 Oct 1;400(4):593-8. doi: