Anti-MSH2 antibody
| Name | Anti-MSH2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70270 |
| Prices | $394.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP IHC-P |
| Species Reactivities | Mouse, Human, Guinea Pig, Bovine, Pig, Monkey, Ape, Monkey, Marmoset, Orangutan, Elephant |
| Antigen | Synthetic peptide: LEREQGEKII QEFLSKVKQM PFTEMSEENI TIKLKQLKAE VIAKNNSFVN EIISRIKVTT , corresponding to C terminal amino acids 875-934 of Human MSH2 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | MSH2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node (left) and mouse squamous cell carcinoma (right) tissues labelling MSH2 with ab70270 at 1/1000 (1µg/ml) and 1/5000 (0.2µg/ml). Detection: DAB.
All lanes : Anti-MSH2 antibody (ab70270) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : Ramos whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µgdeveloped using the ECL technique
Immunoprecipitation of HeLa whole cell lysate. Lane 1: 50µg of input lysate. Lane 2: HeLa whole cell lysate (1mg) immunoprecipitated with ab70270 at 3µg/mg. Lane 3: HeLa whole cell lysate immunoprecipitated with control IgG. Samples were subjected to Western blot, analysed with ab70270 at 0.1µg/ml and detected by chemiluminescence with an exposure time of 3 minutes.
ab70270 staining MSH2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.See Abreview
ICC/IF image of ab70270 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70270, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
X inactivation plays a major role in the gender bias in somatic expansion in a - X inactivation plays a major role in the gender bias in somatic expansion in a
Adihe Lokanga R, Zhao XN, Entezam A, Usdin K. Hum Mol Genet. 2014 Sep 15;23(18):4985-94.
Msh2 acts in medium-spiny striatal neurons as an enhancer of CAG instability and - Msh2 acts in medium-spiny striatal neurons as an enhancer of CAG instability and
Kovalenko M, Dragileva E, St Claire J, Gillis T, Guide JR, New J, Dong H, Kucherlapati R, Kucherlapati MH, Ehrlich ME, Lee JM, Wheeler VC. PLoS One. 2012;7(9):e44273.