MSH2

Gene Symbol MSH2
Entrez Gene 4436
Alt Symbol COCA1, FCC1, HNPCC, HNPCC1, LCFS2
Species Human
Gene Type protein-coding
Description mutS homolog 2
Other Description DNA mismatch repair protein Msh2|hMSH2|mutS homolog 2, colon cancer, nonpolyposis type 1
Swissprots O75488 P43246 B4E2Z2
Accessions AAA82080 AAC27930 AAD14821 AAQ23475 AAS99351 AAY15096 ABQ45882 ABQ45885 ABQ45886 EAX00213 EAX00214 EAX00215 P43246 AK222860 BAD96580 AK223284 BAD97004 AK296831 BAG59401 AK297763 BAG60111 AK299667 BAG61581 AK304496 BAG65304 AK310679 AK311330 BC001122 BC012599 BC021566 AAH21566 BX649122 CB250419 DC342099 DQ648894 ABG49489 DQ648895 ABG49490 DQ648896 ABG49491 DQ648897 ABG49492 DQ892558 ABM83484 DQ895772 ABM86698 L47574 AAB59566 L47575 AAB59567 L47576 AAB59568 L47577 AAB59569 L47578 AAB59570 L47579 AAB59571 L47580 AAB59572 L47581 AAA76858 L47582 AAB59565 L47583 AAB59564 U03911 AAA18643 U04045 AAA61870 XM_005264332 XP_005264389 XM_011532867 XP_011531169 XR_939685 NM_000251 NP_000242 NM_001258281 NP_001245210
Function Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformationa
Subcellular Location Nucleus {ECO:0000305}.
Tissue Specificity Ubiquitously expressed. {ECO:0000269|PubMed:10856833}.
Top Pathways Mismatch repair, Colorectal cancer

MSH2 (D24B5) XP ® Rabbit mAb - 2017 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D24B5
Western blot analysis of extracts of HeLa and NIH/3T3 cells using MSH2 (D24B5) XP ® Rabbit mAb.

MSH2 (3A2) Mouse mAb - 2850 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 3A2
Western blot analysis of extracts of HeLa and OVCAR3 cells using MSH2 (3A2) Mouse mAb.

MSH2 (D-6) - sc-376384 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MSH2 (D-6): sc-376384. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

MSH2 (E-7) - sc-365052 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MSH2 (E-7): sc-365052. Western blot analysis of MSH2 expression in 293 whole cell lysate (A) and HeLa (B) and Hep G2 (C) nuclear extracts.

MSH2 (G-20) - sc-18389 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MSH2 (G-20): sc-18389. Western blot analysis of MSH2 expression in MM-142 nuclear extract.

MSH2 (G-7) - sc-376501 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MSH2 (G-7): sc-376501. Western blot analysis of MSH2 expression in A-431 (A), HeLa (B) and EOC 20 (C) whole cell lysates and IMR-32 nuclear extract (D).

MSH2 (H-300) - sc-22771 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MSH2 (H-300): sc-22771. Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharinx tissue showing nuclear staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

MSH2 (N-19) - sc-18388 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MSH2 (N-19): sc-18388. Western blot analysis of MSH2 expression in A-431 nuclear extract.

MSH2 (N-20) - sc-494 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MSH2 (N-20): sc-494. Western blot analysis of MSH2 expression in A-431 (A) and MM-142 (B) whole cell lysates.

MSH2 (25D12) - sc-56163 from Santa Cruz Biotechnology

Prices $279.00
Sizes 250 µl supernatant
Host Mouse
Clonality Monoclonal
MSH2 (25D12): sc-56163. Western blot analysis of MSH2 expression in A-431 (A) and SW480 (B) whole cell lysates and A-431 (C), U-937 (D), MOLT-4 (E) and SW480 (F) nuclear extracts.

Anti-MSH2 antibody - ab70270 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node (left) and mouse squamous cell carcinoma (right) tissues labelling MSH2 with ab70270 at 1/1000 (1µg/ml) and 1/5000 (0.2µg/ml). Detection: DAB.

Anti-MSH2 antibody - ab84421 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon adenocarcinoma using 1/250 ab84420, followed by DAB staining.

Anti-MSH2 antibody - ab9146 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-MSH2 antibody - ab139318 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-MSH2 antibody (ab139318) at 1/500 dilution + A431 total cell extract

Anti-MSH2 antibody - ab47076 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab47076 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47076, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MSH2 antibody [1184CT1.3.2] - ab188381 from Abcam

Prices $376.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Clone 1184CT1.3.2
All lanes : Anti-MSH2 antibody [1184CT1.3.2] (ab188381) at 1/2000 dilutionLane 1 : 293 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 35 µg per lane.

Anti-MSH2 antibody [25D12] - ab2353 from Abcam

Prices $376.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone 25D12
Anti-MSH2 antibody [25D12] (ab2353) at 1/500 dilution + JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Anti-MSH2 antibody [3A2B8C] - ab52266 from Abcam

Prices $403.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A2B8C
ab52266 (1/200) detecting MSH2 in HeLa cells (green). Cells were fixed in methanol (-20'C, 10min) and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.See Abreview

Anti-MSH2 antibody (Agarose) - ab1294 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MSH2 antibody [EPR3943] - ab92473 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3943
All lanes : Anti-MSH2 antibody [EPR3943] (ab92473) at 1/1000 dilutionLane 1 : A375 cell lysateLane 2 : A431 cell lysateLane 3 : SW480 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit antibody at 1/2000 dilution

Anti-MSH2 antibody [EPR3944] - ab133330 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3944
All lanes : Anti-MSH2 antibody [EPR3944] (ab133330) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : A375 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-MSH2 antibody, prediluted - ab70640 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Human colon carcinoma tissue stained with undiluted ab70640 for 30 min at RT.

Anti-MSH2 antibody [SP46], prediluted - ab101689 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Monoclonal
Clone SP46
Flow cytometric analysis of rabbit anti-MSH2 (SP46) antibody, prediluted ab101689  in HeLa  cells(green) compare to negative control of rabbit IgG (blue)

Anti-MSH2 antibody - ChIP Grade - ab16833 from Abcam

Prices $369.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MSH2 antibody - ChIP Grade (ab16833) at 1/500 dilution + Human Glioblastoma whole cell lysateSecondaryHRP-conjugated Goat anti-Rabbit at 1/7500 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MSH2 Antibody - LS-C9095 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
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