Anti-MSH2 antibody [3A2B8C]
| Name | Anti-MSH2 antibody [3A2B8C] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52266 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 3A2B8C |
| Applications | FC ICC/IF ICC/IF IHC-F WB IHC-P ELISA IP |
| Species Reactivities | Human, Monkey |
| Antigen | Ni-NTA purified recombinant human MSH2 expressed in E |
| Description | Mouse Monoclonal |
| Gene | MSH2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab52266 (1/200) detecting MSH2 in HeLa cells (green). Cells were fixed in methanol (-20'C, 10min) and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.See Abreview
![All lanes : Anti-MSH2 antibody [3A2B8C] (ab52266) at 1/2000 dilutionLane 1 : Cell lysates prepared from human Hela cells Lane 2 : Cell lysates prepared from A549 cellsLane 3 : Cell lysates prepared from human A431 cellsLane 4 : Cell lysates prepared from HEK293 cellsLysates/proteins at 100 µg per lane.SecondaryHRP-conjugated Goat polyclonal to mouse IgG](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/25019_MSH2-Primary-antibodies-ab52266-2.jpg)
All lanes : Anti-MSH2 antibody [3A2B8C] (ab52266) at 1/2000 dilutionLane 1 : Cell lysates prepared from human Hela cells Lane 2 : Cell lysates prepared from A549 cellsLane 3 : Cell lysates prepared from human A431 cellsLane 4 : Cell lysates prepared from HEK293 cellsLysates/proteins at 100 µg per lane.SecondaryHRP-conjugated Goat polyclonal to mouse IgG
ab52266 at 1/1000 dilution staining MSH2 in human Hela cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG1 was used as secondary antibody. The primary antibody shows green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 phalloidin.
![Overlay histogram showing HeLa cells stained with ab52266 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52266, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/25021_MSH2-Primary-antibodies-ab52266-4.jpg)
Overlay histogram showing HeLa cells stained with ab52266 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52266, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
MSH2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Mouse monoclonal to MSH2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52266.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/10,000 dilution.Band: 105kDa; MSH2
Immunohistochemical analysis of paraffin-embedded human rectum carcinoma tissue, showing nuclear and cytoplasmic localisation, using ab52266 at a dilution of 1/200 - 1/1000 with DAB staining.
Product References
Human embryonic stem cells show low-grade microsatellite instability. - Human embryonic stem cells show low-grade microsatellite instability.
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Identification of proteins at active, stalled, and collapsed replication forks - Identification of proteins at active, stalled, and collapsed replication forks
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Role of mismatch repair enzymes in GAA.TTC triplet-repeat expansion in Friedreich - Role of mismatch repair enzymes in GAA.TTC triplet-repeat expansion in Friedreich
Du J, Campau E, Soragni E, Ku S, Puckett JW, Dervan PB, Gottesfeld JM. J Biol Chem. 2012 Aug 24;287(35):29861-72.
UNG-initiated base excision repair is the major repair route for 5-fluorouracil - UNG-initiated base excision repair is the major repair route for 5-fluorouracil
Pettersen HS, Visnes T, Vagbo CB, Svaasand EK, Doseth B, Slupphaug G, Kavli B, Krokan HE. Nucleic Acids Res. 2011 Oct;39(19):8430-44.
Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and is required for - Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and is required for
Svendsen JM, Smogorzewska A, Sowa ME, O'Connell BC, Gygi SP, Elledge SJ, Harper JW. Cell. 2009 Jul 10;138(1):63-77.