Anti-MSH2 antibody - ChIP Grade
| Name | Anti-MSH2 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab16833 |
| Prices | $369.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F IP IHC-P ChIP |
| Species Reactivities | Mouse, Human |
| Antigen | Full length recombinant MSH2 protein |
| Description | Rabbit Polyclonal |
| Gene | MSH2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-MSH2 antibody - ChIP Grade (ab16833) at 1/500 dilution + Human Glioblastoma whole cell lysateSecondaryHRP-conjugated Goat anti-Rabbit at 1/7500 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of ab16833 staining in human seminoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16833, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
An inverse switch in DNA base excision and strand break repair contributes to - An inverse switch in DNA base excision and strand break repair contributes to
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MutSbeta and histone deacetylase complexes promote expansions of trinucleotide - MutSbeta and histone deacetylase complexes promote expansions of trinucleotide
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Loss of Rad51c leads to embryonic lethality and modulation of Trp53-dependent - Loss of Rad51c leads to embryonic lethality and modulation of Trp53-dependent
Kuznetsov SG, Haines DC, Martin BK, Sharan SK. Cancer Res. 2009 Feb 1;69(3):863-72.
Limiting the persistence of a chromosome break diminishes its mutagenic - Limiting the persistence of a chromosome break diminishes its mutagenic
Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM. PLoS Genet. 2009 Oct;5(10):e1000683.