Anti-mSin3A antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab3479
• Prices: $395.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF ChIP ChIPseq IP WB IHC-P
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide corresponding to Mouse mSin3A aa 1-19
• Description: Rabbit Polyclonal
• Gene: SIN3A
• Conjugate: Unconjugated

Product images

ab3479 (2µg/ml) staining mSin3A in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclei of epithelial cells.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1/ EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Product References

LATS2 is a modulator of estrogen receptor alpha. - LATS2 is a modulator of estrogen receptor alpha.

Lit LC, Scott S, Zhang H, Stebbing J, Photiou A, Giamas G. Anticancer Res. 2013 Jan;33(1):53-63.

Ten-eleven translocation 1 (Tet1) is regulated by O-linked N-acetylglucosamine - Ten-eleven translocation 1 (Tet1) is regulated by O-linked N-acetylglucosamine

Shi FT, Kim H, Lu W, He Q, Liu D, Goodell MA, Wan M, Songyang Z. J Biol Chem. 2013 Jul 19;288(29):20776-84.

Exercise and diabetes have opposite effects on the assembly and O-GlcNAc - Exercise and diabetes have opposite effects on the assembly and O-GlcNAc

Cox EJ, Marsh SA. Cardiovasc Diabetol. 2013 Jul 9;12:101.

The C-terminal putative nuclear localization sequence of breast cancer metastasis - The C-terminal putative nuclear localization sequence of breast cancer metastasis

Hurst DR, Xie Y, Thomas JW, Liu J, Edmonds MD, Stewart MD, Welch DR. PLoS One. 2013;8(2):e55966.

Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC - Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC

Bantscheff M, Hopf C, Savitski MM, Dittmann A, Grandi P, Michon AM, Schlegl J, Abraham Y, Becher I, Bergamini G, Boesche M, Delling M, Dumpelfeld B, Eberhard D, Huthmacher C, Mathieson T, Poeckel D, Reader V, Strunk K, Sweetman G, Kruse U, Neubauer G, Ramsden NG, Drewes G. Nat Biotechnol. 2011 Mar;29(3):255-65.

TET1 and hydroxymethylcytosine in transcription and DNA methylation fidelity. - TET1 and hydroxymethylcytosine in transcription and DNA methylation fidelity.

Williams K, Christensen J, Pedersen MT, Johansen JV, Cloos PA, Rappsilber J, Helin K. Nature. 2011 May 19;473(7347):343-8.

Functional significance of mutations in the Snf2 domain of ATRX. - Functional significance of mutations in the Snf2 domain of ATRX.

Mitson M, Kelley LA, Sternberg MJ, Higgs DR, Gibbons RJ. Hum Mol Genet. 2011 Jul 1;20(13):2603-10.

Silencing of GSTP1, a prostate cancer prognostic gene, by the estrogen - Silencing of GSTP1, a prostate cancer prognostic gene, by the estrogen

Re A, Aiello A, Nanni S, Grasselli A, Benvenuti V, Pantisano V, Strigari L, Colussi C, Ciccone S, Mazzetti AP, Pierconti F, Pinto F, Bassi P, Gallucci M, Sentinelli S, Trimarchi F, Bacchetti S, Pontecorvi A, Lo Bello M, Farsetti A. Mol Endocrinol. 2011 Dec;25(12):2003-16.

Deacetylase inhibitors dissociate the histone-targeting ING2 subunit from the - Deacetylase inhibitors dissociate the histone-targeting ING2 subunit from the

Smith KT, Martin-Brown SA, Florens L, Washburn MP, Workman JL. Chem Biol. 2010 Jan 29;17(1):65-74.

T-bet dependent removal of Sin3A-histone deacetylase complexes at the Ifng locus - T-bet dependent removal of Sin3A-histone deacetylase complexes at the Ifng locus

Chang S, Collins PL, Aune TM. J Immunol. 2008 Dec 15;181(12):8372-81.