Anti-MSK1 (phospho S360) antibody [EP1888Y]

• Supplier: Abcam
• Catalog: ab81294
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1888Y
• Applications: ICC/IF IP WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic phospho peptide corresponding to residues surrounding Serine 360 of human MSK1
• Description: Rabbit Monoclonal
• Gene: RPS6KA5
• Conjugate: Unconjugated

Product images

All lanes : Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294) at 1/20000 dilutionLane 1 : HEK293 cell lyasate, untreated,Lane 2 : HEK293 cell lyasate treated with AP.Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti rabbit. at 1/2000 dilution

ICC/IF image of ab81294 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81294, neat) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Mechanisms of dopamine D1 receptor-mediated ERK1/2 activation in the parkinsonian - Mechanisms of dopamine D1 receptor-mediated ERK1/2 activation in the parkinsonian

Fieblinger T, Sebastianutto I, Alcacer C, Bimpisidis Z, Maslava N, Sandberg S, Engblom D, Cenci MA. J Neurosci. 2014 Mar 26;34(13):4728-40.

Dihydrotestosterone stimulates amino acid uptake and the expression of LAT2 in - Dihydrotestosterone stimulates amino acid uptake and the expression of LAT2 in

Hamdi MM, Mutungi G. J Physiol. 2011 Jul 15;589(Pt 14):3623-40.