RPS6KA5

Gene Symbol RPS6KA5
Entrez Gene 9252
Alt Symbol MSK1, MSPK1, RLPK
Species Human
Gene Type protein-coding
Description ribosomal protein S6 kinase, 90kDa, polypeptide 5
Other Description 90 kDa ribosomal protein S6 kinase 5|RSK-like protein kinase|RSKL|S6K-alpha-5|nuclear mitogen- and stress-activated protein kinase 1|ribosomal protein S6 kinase alpha-5
Swissprots O95316 O75582 B7Z2Y5 Q96AF7
Accessions EAW81439 EAW81440 O75582 AF074393 AAC31171 AF080000 AAD23915 AF090421 AAC69577 AK295266 BAH12021 AK309925 AL050099 CAB43270 BC017187 AAH17187 BF593074 BG699153 XM_011537360 XP_011535662 NM_004755 NP_004746 NM_182398 NP_872198
Function Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential. Phos
Subcellular Location Nucleus. Cytoplasm. Note=Predominantly nuclear. Exported into cytoplasm in response to glucocorticoid.
Tissue Specificity Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. {ECO:0000269|PubMed:9687510, ECO:0000269|PubMed:9873047}.
Top Pathways Bladder cancer, MAPK signaling pathway, Neurotrophin signaling pathway, Circadian entrainment, TNF signaling pathway

Phospho-MSK1 (Ser376) Antibody - 9591 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from RSK1, RSK2, RSK3 or MSK1 transfected 293 cells, untreated or TPA-treated (200 nM), using Phospho-MSK1 (Ser376) Antibody.

Phospho-MSK1 (Thr581) Antibody - 9595 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, transfected with wt MSK1 or mutant MSK1, untreated or TPA-treated (200 nM), using Phospho-MSK1 (Thr581) Antibody.

Phospho-MSK1 (Ser360) Antibody - 9594 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells transfected with MSK1 or various mutants of MSK1, then treated with TPA (200 nM) and analyzed using Phospho-MSK1 (Ser360) Antibody.

MSK1 (C27B2) Rabbit mAb - 3489 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C27B2
Western blot analysis of extracts from various cell lines using MSK1 (C27B2) Rabbit mAb.

MSK1 (H-19) - sc-9392 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MSK1 (H-19): sc-9392. Western blot analysis of MSK1 expression in HL-60 (A) and HeLa (B) whole cell lysates.

MSK1 (H-65) - sc-25417 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MSK1 (H-65): sc-25417. Western blot analysis of MSK1 expression in UV-induced HeLa (A) and JAR (B) whole cell lysates.

MSK1 (N-18) - sc-9391 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MSK1 (N-18): sc-9391. Western blot analysis of MSK1 expression in non-transfected: sc-110760 (A) and human MSK1 transfected: sc-158744 (B) 293 whole cell lysates.

MSK1 (D-77) - sc-130431 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

p-MSK1 (Ser 360) - sc-130205 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-MSK1 (Ser 360): sc-130205. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.

Anti-MSK1 (phospho S212) antibody - ab79499 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MSK1 (phospho S212) antibody (ab79499) at 1/1000 dilutionLane 1 : extracts from 3T3 cells (treated with EGF at 200ng/ml for 5 mins)Lane 2 : extracts from 3T3 cells (treated with EGF at 200ng/ml for 5 mins) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-MSK1 (phospho S212) antibody - ab192451 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MSK1 (phospho S212) antibody (ab192451) at 1/500 dilutionLane 1 : EGF treated NIH 3T3 whole cell lysates with antigen-specific peptideLane 2 : EGF treated NIH 3T3 whole cell lysatesLysates/proteins at 12.5 µg per lane.developed using the ECL technique

Anti-MSK1 (phospho S360) antibody - ab62201 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab62201 at 1/50 dilution. Right hand panel was treated with immunising phosphopeptide.

Anti-MSK1 (phospho S360) antibody [EP1888Y] - ab81294 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1888Y
All lanes : Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294) at 1/20000 dilutionLane 1 : HEK293 cell lyasate, untreated,Lane 2 : HEK293 cell lyasate treated with AP.Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti rabbit. at 1/2000 dilution

Anti-MSK1 (phospho S376) antibody - ab195933 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MSK1 (phospho S376) antibody (ab195933) at 1/500 dilutionLane 1 : HeLa whole cell lysate with antigen-specific peptideLane 2 : HeLa whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-MSK1 (phospho S376) antibody - ab51041 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MSK1 (phospho S376) antibody (ab51041) at 1/500 dilutionLane 1 : Hela whole cell lysate, cells treated with PMA (125ng/ml for 15minutes)Lane 2 : Hela whole cell lysate

Anti-MSK1 (phospho S376) antibody [E375] - ab32190 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E375
All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/5000 dilutionLane 1 : Untreated K562 cell lysateLane 2 : K562 cell lysate treated with Alkaline

Anti-MSK1 (phospho S376) antibody [E375] (Alexa Fluor® 647) - ab201263 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E375
ab201263 staining MSK1 (phospho S376) in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201263 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 4% formaldehyde (10 min).

Anti-MSK1 (phospho T581) antibody - ab59336 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MSK1 (phospho T581) antibody (ab59336) at 1/500 dilutionLane 1 : Extracts from RAW264.7 cells, treated with UV (5 mins) with no immunizing peptide Lane 2 : Extracts from RAW264.7 cells, treated with UV (5 mins) with immunizing peptideObserved band size : 90 kDa (why is the actual band size different from the predicted?)

Anti-MSK1 (phospho T581) antibody - ab2562 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-MSK1 antibody - ab63619 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of HuvEc cells, using ab63619 at a dilution of 1/500-1/1000. Samples were untreated (left hand panel) or treated (right hand panel) with the immunizing peptide.

Anti-MSK1 antibody - ab155405 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MSK1 antibody (ab155405) at 1/500 dilutionLane 1 : 293 cells treated with UV for 15 minutesLane 2 : 293 cells treated with UV for 15 minutes with synthesised peptide

Anti-MSK1 antibody - ab99412 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MSK1 with ab99421 at 1/200 (1µg/mg). Detection: DAB.

Anti-MSK1 antibody - ab133199 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human small intestine tissue labelling MSK1 with ab133199 at 10 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-MSK1 antibody - ab59296 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Ab59296 at 1/50 dilution staining human pancreas tissue without (left) and with (right) immunizing peptide; paraffin embedded.

Anti-RPS6KA5 / MSK1 Antibody (aa1-802) - LS-C16608 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
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