Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MSK1 with ab99421 at 1/200 (1µg/mg). Detection: DAB.
All lanes : Anti-MSK1 antibody (ab99412) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T cell lysate at 50 µgdeveloped using the ECL technique
Detection of MSK1 in immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded on gel) using ab99412 at 3 µg/mg lysate for IP, and at 1 µg/ml for the subsequent western blot. Detection: chemiluminescence with exposure time of 10 seconds.