Anti-MSK2 / RSK-B antibody
| Name | Anti-MSK2 / RSK-B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab42101 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human MSK2/ RSK-B |
| Description | Rabbit Polyclonal |
| Gene | RPS6KA4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-MSK2 / RSK-B antibody (ab42101) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab42101 stained MCF7 cells. The cells were 4%PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42101, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2 cells fixed in 4%PFA at 1ug/ml and HeLa, Hek293, HepG2 and MCF7 cells fixed in 100% methanol at 5ug/ml.
Product References
HCV-induced PKR activation is stimulated by the mitogen- and stress-activated - HCV-induced PKR activation is stimulated by the mitogen- and stress-activated
Kang JI, Ahn BY. Biochem Biophys Res Commun. 2011 Apr 1;407(1):248-53. doi:
Anthrax lethal toxin impairs IL-8 expression in epithelial cells through - Anthrax lethal toxin impairs IL-8 expression in epithelial cells through
Raymond B, Batsche E, Boutillon F, Wu YZ, Leduc D, Balloy V, Raoust E, Muchardt C, Goossens PL, Touqui L. PLoS Pathog. 2009 Apr;5(4):e1000359.