Anti-MST3 antibody [EP1468Y]
| Name | Anti-MST3 antibody [EP1468Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab51137 |
| Prices | $357.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1468Y |
| Applications | FC IP WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues on the C-terminus of human MST3 |
| Description | Rabbit Monoclonal |
| Gene | STK24 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-MST3 antibody [EP1468Y] (ab51137) at 1/5000 dilution + HeLa cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/25225_ab51137_1.bmp)
Anti-MST3 antibody [EP1468Y] (ab51137) at 1/5000 dilution + HeLa cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution
Ab51137 (1:100) staining human MST3 in human brain tissue by immunohistochemistry using paraffin embedded tissue.
Immunofluorescent staining of HeLa cells using ab51137 (1:100).
Overlay histogram showing HeLa cells stained with ab51137 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51137, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
Cdk5-dependent Mst3 phosphorylation and activity regulate neuronal migration - Cdk5-dependent Mst3 phosphorylation and activity regulate neuronal migration
Tang J, Ip JP, Ye T, Ng YP, Yung WH, Wu Z, Fang W, Fu AK, Ip NY. J Neurosci. 2014 May 28;34(22):7425-36.
STK25 protein mediates TrkA and CCM2 protein-dependent death in pediatric tumor - STK25 protein mediates TrkA and CCM2 protein-dependent death in pediatric tumor
Costa B, Kean MJ, Ast V, Knight JD, Mett A, Levy Z, Ceccarelli DF, Badillo BG, Eils R, Konig R, Gingras AC, Fainzilber M. J Biol Chem. 2012 Aug 24;287(35):29285-9.
Protein phosphatase 2a (PP2A) binds within the oligomerization domain of striatin - Protein phosphatase 2a (PP2A) binds within the oligomerization domain of striatin
Gordon J, Hwang J, Carrier KJ, Jones CA, Kern QL, Moreno CS, Karas RH, Pallas DC. BMC Biochem. 2011 Oct 10;12:54.
CCM3 signaling through sterile 20-like kinases plays an essential role during - CCM3 signaling through sterile 20-like kinases plays an essential role during
Zheng X, Xu C, Di Lorenzo A, Kleaveland B, Zou Z, Seiler C, Chen M, Cheng L, Xiao J, He J, Pack MA, Sessa WC, Kahn ML. J Clin Invest. 2010 Aug;120(8):2795-804.