Anti-MTAP antibody (ab96231) at 1/1000 dilution + A431 whole cell lysate at 30 µg
ab96231, at a 1/500 dilution, staining Human MTAP in SAS xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
ab96231 staining MTAP in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized; antigen retrieval was by heat mediation in Tris-EDTA buffer, pH 8.5. Samples were incubated with primary antibody (1/500) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview