Western blot analysis of U2OS cells extracts immunoprecipitated using ab197855Lane 1: Input.Lane 2: Immunoprecipitation with control serum.Lane 3: Immunoprecipitation with ab197855 (5ul)
All lanes : Anti-MTH1 antibody (ab197855) at 1/2000 dilutionLane 1 : Control siRNA transfected U2OS cell lysateLane 2 : MTH1 siRNA transfected U2OS cell lysateLane 3 : NIH3T3 cell lysateLysates/proteins at 40 µg per lane.
Immunofluorescent analysis of PFA fixed and methanol permeabilized U2OS cells using ab197855 at 1/600 dilution in PBST (Green). DAPI staining shown in blue.