Anti-MTH1 antibody [EPR15934]

• Supplier: Abcam
• Catalog: ab197028
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15934
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Human, Mouse, Rat
• Antigen: Recombinant fragment within Human MTH1 aa 50-150
• Description: Rabbit Monoclonal
• Gene: NUDT1
• Conjugate: Unconjugated

Product images

All lanes : Anti-MTH1 antibody [EPR15934] (ab197028) at 1/2000 dilutionLane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 2 : A549 (Human lung carcinoma) whole cell lysateLane 3 : Human fetal thymus lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling MTH1 with ab197028 at 1/100 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasm and nuclear staining on Human spleen is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody. 

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-3 cells (Human prostate cancer cell line)  labeling MTH1 with ab197028 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm and nuclear staining on PC-3 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:--ve control 1 - ab197028 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.