Anti-MTR antibody
| Name | Anti-MTR antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab66039 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, C. elegans, Horse, Pig, Chimpanzee |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1200 to the C-terminus of Human MTR |
| Description | Rabbit Polyclonal |
| Gene | MTR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-MTR antibody (ab66039) at 1 µg/mlLane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell LysateLane 2 : Liver (Mouse) Tissue LysateLane 3 : Thymus (Rat) Tissue LysateLane 4 : Pancreas (Mouse) Tissue LysateLane 5 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)Lane 6 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)Lane 7 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 9 : Kidney (Mouse) Tissue LysateLane 10 : Liver (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab66039 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66039, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 5µg/ml, and in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.
IHC image of ab66039 staining in pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66039, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
A dodecylamine derivative of cyanocobalamin potently inhibits the activities of - A dodecylamine derivative of cyanocobalamin potently inhibits the activities of
Bito T, Yabuta Y, Ichiyanagi T, Kawano T, Watanabe F. FEBS Open Bio. 2014 Aug 1;4:722-9.
Levels of key enzymes of methionine-homocysteine metabolism in preeclampsia. - Levels of key enzymes of methionine-homocysteine metabolism in preeclampsia.
Perez-Sepulveda A, Espana-Perrot PP, Fernandez XB, Ahumada V, Bustos V, Arraztoa JA, Dobierzewska A, Figueroa-Diesel H, Rice GE, Illanes SE. Biomed Res Int. 2013;2013:731962.
Melanoma coordinates general and cell-specific mechanisms to promote methotrexate - Melanoma coordinates general and cell-specific mechanisms to promote methotrexate
Saez-Ayala M, Fernandez-Perez MP, Montenegro MF, Sanchez-del-Campo L, Chazarra S, Pinero-Madrona A, Cabezas-Herrera J, Rodriguez-Lopez JN. Exp Cell Res. 2012 Jun 10;318(10):1146-59.
Improving drug potency and efficacy by nanocarrier-mediated subcellular - Improving drug potency and efficacy by nanocarrier-mediated subcellular
Murakami M, Cabral H, Matsumoto Y, Wu S, Kano MR, Yamori T, Nishiyama N, Kataoka K. Sci Transl Med. 2011 Jan 5;3(64):64ra2.