Anti-mtTFA antibody
| Name | Anti-mtTFA antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab47517 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human mtTFA |
| Description | Rabbit Polyclonal |
| Gene | TFAM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-mtTFA antibody (ab47517) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab47517 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab47517, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-mtTFA antibody (ab47517) at 2 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human mtTFA peptide (ab47516) at 2 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
IHC-P image of mtTFA staining using ab47517 in kidney (upper) and Colon (lower) sections. The tissue was fixed using formaldehyde. Heat mediated epitope retrieval was carried out using citric acid (pH 6). The sections were blocked uwing 1% BSA for 10 mins at 21°C.See Abreview
Product References
Dynamic regulation of metabolic efficiency explains tolerance to acute hypoxia in - Dynamic regulation of metabolic efficiency explains tolerance to acute hypoxia in
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Mitochondrial loss, dysfunction and altered dynamics in Huntington's disease. - Mitochondrial loss, dysfunction and altered dynamics in Huntington's disease.
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Role of tyrosyl-DNA phosphodiesterase (TDP1) in mitochondria. - Role of tyrosyl-DNA phosphodiesterase (TDP1) in mitochondria.
Das BB, Dexheimer TS, Maddali K, Pommier Y. Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19790-5. doi: