TFAM

Gene Symbol TFAM
Entrez Gene 7019
Alt Symbol MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3
Species Human
Gene Type protein-coding
Description transcription factor A, mitochondrial
Other Description mitochondrial transcription factor 1|mitochondrial transcription factor A|transcription factor 6|transcription factor 6-like 1|transcription factor 6-like 2 (mitochondrial transcription factor)|transcription factor 6-like 3
Swissprots A9QXC6 Q5U0C6 Q00059 B5BU05 A8MRB2
Accessions ADP91974 ADP91975 ADP91976 ADP91977 ADP91978 ADP91979 ADP91980 ADP91981 ADP91982 ADP91983 ADP91984 ADP91985 ADP91986 ADP91987 ADP91988 ADP91989 ADP91990 ADP91991 ADP91992 ADP91993 ADP91994 ADP91995 ADP91996 ADP91997 ADP91998 ADP91999 ADP92000 ADP92001 ADP92002 ADP92003 ADP92004 ADP92005 ADP92006 ADP92007 ADP92008 ADP92009 ADP92010 ADP92011 ADP92012 ADP92013 CAA45562 EAW54171 EAW54172 EAW54173 EAW54174 EAW54175 Q00059 AB451241 BAG70055 AB451366 BAG70180 AK312558 BAG35455 BC018628 AAH18628 BC029815 BC104482 AAI04483 BC126366 AAI26367 BM455062 BT019658 AAV38464 BT019659 AAV38465 CN478914 CR407653 CAG28581 CR736151 DB456976 EU279428 ABX72056 HQ258758 ADR83509 M62810 AAA59849 XM_011540120 XP_011538422 XM_011540121 XP_011538423 NM_001270782 NP_001257711 NM_003201 NP_003192 NR_073073 NM_012251
Function Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. {ECO:0000269|PubMed:1737790, ECO:0000269|PubMed:19304746, ECO:0000269|PubMed:20410300, ECO:0000269|PubMed:22037171, ECO:0000269|PubMed:22037172, ECO:0000269|PubMed:22841477}.
Subcellular Location Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid.
Top Pathways Huntington's disease

TFAM Antibody - 7495 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and 293T cells using TFAM Antibody.

TFAM (D5C8) Rabbit mAb - 8076 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5C8
Western blot analysis of extracts from HeLa and A172 cells using TFAM (D5C8) Rabbit mAb.

mtTFA (C-9) - sc-376672 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
mtTFA (C-9): sc-376672. Western blot analysis of mtTFA expression in A-431 (A), CCRF-CEM (B) and HeLa (C) whole cell lysates.

mtTFA (E-16) - sc-30963 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
mtTFA (E-16): sc-30963. Western blot analysis of mtTFA expression in A-431 (A), CCRF-CEM (B), HeLa (C) and NTERA-2 cl.D1 (D) whole cell lysates.

mtTFA (A-16) - sc-30962 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
mtTFA (A-16): sc-30962. Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.

mtTFA (H-203) - sc-28200 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
mtTFA (H-203): sc-28200. Western blot analysis of mtTFA expression in 293T whole cell lysate.

mtTFA (K-18) - sc-19050 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
mtTFA (K-18): sc-19050. Western blot analysis of mtTFA expression in A-431 (A) and CCRF-CEM (B) whole cell lysates.

Anti-mtTFA antibody - ab47517 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-mtTFA antibody (ab47517) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-mtTFA antibody - ab89818 from Abcam

Prices $390.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-mtTFA antibody (ab89818) at 1 µg/ml + Human kidney lysate at 50 µg

Anti-mtTFA antibody - ab180763 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling mtTFA with ab180763 at 1/100. Magnification: 200x.

Anti-mtTFA antibody - ab155240 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of methanol-fixed HeLa cells labeling mtTFA using ab155240 antibody at 1/500 dilution.

Anti-mtTFA antibody - ab138351 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-mtTFA antibody (ab138351) at 1 µg/ml (Milk blocking 1%)Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-mtTFA antibody - ab114993 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-mtTFA antibody (ab114993) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-mtTFA antibody - ab119294 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab119294 at 10 &microg/ml staining mtTFA in Human Skeletal Muscle by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

Anti-mtTFA antibody - ab114984 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of mtTFA by Western Blot of Immunprecipitate. Anti-mtTFA at 1µg/ml staining mtTFA in HeLa whole cell lysate immunoprecipitated using ab114984 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-mtTFA antibody [18G102B2E11] - ab119684 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 18G102B2E11
Immunocytochemistry image of ab119684 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (18G102B2E11, 1µg/ml) for 2h at room temperature. The secondary antibody was (green) 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). mtTFA localizes to the mitochondria.

Anti-mtTFA antibody [18G102B2E11] (Alexa Fluor® 488) - ab198308 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 18G102B2E11
ab198308 staining mtTFA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab198308 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-mtTFA antibody [18G102B2E11] (Alexa Fluor® 647) - ab198106 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 18G102B2E11
ab198106 staining mtTFA in WI38 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198106 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-mtTFA antibody [18G102B2E11] (HRP) - ab198307 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 18G102B2E11
Anti-mtTFA antibody [18G102B2E11] (HRP) (ab198307) at 1/5000 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035) at 20 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-mtTFA antibody [EPR12285] - ab176558 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12285
All lanes : Anti-mtTFA antibody [EPR12285] (ab176558) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : MCF7 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.developed using the ECL technique

Anti-mtTFA antibody [EPR12286] - ab171951 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12286
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling mtTFA with ab171951 at 1/100 dilution.

Anti-TFAM antibody - ab131607 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-TFAM antibody (ab131607) at 1/2000 dilution + Rat kidney lysate

Anti-MtTFA / TFAM Antibody (aa43-246) - LS-C296734 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant MtTFA / TFAM.

Anti-MtTFA / TFAM Antibody (clone 18G102B2E11) IHC-plus™ - LS-B9989 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 18G102B2E11
Anti-MtTFA / TFAM antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9989 concentration 10 ug/ml.

Anti-MtTFA / TFAM Antibody (aa151-200) IHC-plus™ - LS-B6950 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-TFAM antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6950 concentration 5 ug/ml.
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