All lanes : Anti-mtTFA antibody (ab138351) at 1 µg/ml (Milk blocking 1%)Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.