All lanes : Anti-mtTFA antibody [EPR12285] (ab176558) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : MCF7 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.developed using the ECL technique
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling mtTFA using ab176558 at a 1/50 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue, labeling mtTFA using ab176558 at a 1/50 dilution.
Immunofluorescence analysis of MCF7 cells, labeling mtTFA using ab176558 at a 1/100 dilution.
Western blot analysis on Immunoprecipitation pellet from either 1) 293T cell lysate, or 2) 1xPBS (negative control), showing mtTFA, using ab176558 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.