Anti-Mu Opioid Receptor antibody [UMB3]

• Supplier: Abcam
• Catalog: ab134054
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: UMB3
• Applications: WB IP IHC-P FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to C terminal residues of Human Mu Opioid Receptor
• Description: Rabbit Monoclonal
• Gene: OPRM1
• Conjugate: Unconjugated

Product images

Overlay histogram showing SH-SY5Y cells stained with ab134054 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134054, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Mu Opioid Receptor antibody [UMB3] (ab134054) at 1/1000 dilutionLane 1 : Mu Opioid Receptor transfected HEK293 lysateLane 2 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Product References

Differentiation of opioid drug effects by hierarchical multi-site - Differentiation of opioid drug effects by hierarchical multi-site

Just S, Illing S, Trester-Zedlitz M, Lau EK, Kotowski SJ, Miess E, Mann A, Doll C, Trinidad JC, Burlingame AL, von Zastrow M, Schulz S. Mol Pharmacol. 2013 Mar;83(3):633-9.

Dopaminergic amacrine cells express opioid receptors in the mouse retina. - Dopaminergic amacrine cells express opioid receptors in the mouse retina.

Gallagher SK, Anglen JN, Mower JM, Vigh J. Vis Neurosci. 2012 May;29(3):203-9.

Analgesic tolerance to high-efficacy agonists but not to morphine is diminished - Analgesic tolerance to high-efficacy agonists but not to morphine is diminished

Grecksch G, Just S, Pierstorff C, Imhof AK, Gluck L, Doll C, Lupp A, Becker A, Koch T, Stumm R, Hollt V, Schulz S. J Neurosci. 2011 Sep 28;31(39):13890-6.

UMB-3, a novel rabbit monoclonal antibody, for assessing mu-opioid receptor - UMB-3, a novel rabbit monoclonal antibody, for assessing mu-opioid receptor

Lupp A, Richter N, Doll C, Nagel F, Schulz S. Regul Pept. 2011 Feb 25;167(1):9-13.

Agonist-selective patterns of micro-opioid receptor phosphorylation revealed by - Agonist-selective patterns of micro-opioid receptor phosphorylation revealed by

Doll C, Konietzko J, Poll F, Koch T, Hollt V, Schulz S. Br J Pharmacol. 2011 Sep;164(2):298-307.