Anti-MUC16 antibody [OC125]
| Name | Anti-MUC16 antibody [OC125] |
|---|---|
| Supplier | Abcam |
| Catalog | ab693 |
| Prices | $382.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | OC125 |
| Applications | FC IHC-P IHC-F |
| Species Reactivities | Human |
| Antigen | Partially purified human mucin fraction from a pool of tissues from patients with epithelial ovarian cancer |
| Description | Mouse Monoclonal |
| Gene | MUC16 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab693 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab693, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/25889_MUC16-Primary-antibodies-ab693-1.jpg)
Overlay histogram showing HeLa cells stained with ab693 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab693, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
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What do we know about the origin of CA 125? - What do we know about the origin of CA 125?
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Immunopathologic characterization of a monoclonal antibody that recognizes common - Immunopathologic characterization of a monoclonal antibody that recognizes common
Kabawat SE, Bast RC, Welch WR, Knapp RC, Colvin RB. Am J Clin Pathol. 1983 Jan;79(1):98-104.
Tissue distribution of a coelomic-epithelium-related antigen recognized by the - Tissue distribution of a coelomic-epithelium-related antigen recognized by the
Kabawat SE, Bast RC Jr, Bhan AK, Welch WR, Knapp RC, Colvin RB. Int J Gynecol Pathol. 1983;2(3):275-85.
Reactivity of a monoclonal antibody with human ovarian carcinoma. - Reactivity of a monoclonal antibody with human ovarian carcinoma.
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