Anti-MUC16 antibody [X75]
| Name | Anti-MUC16 antibody [X75] |
|---|---|
| Supplier | Abcam |
| Catalog | ab1107 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | X75 |
| Applications | WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | CA-125 purified from human ovarian carcinoma |
| Description | Mouse Monoclonal |
| Gene | MUC16 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab1107 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1107, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed HeLa cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/25896_ab1107-2-ab1107FC.jpg)
Overlay histogram showing HeLa cells stained with ab1107 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1107, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed HeLa cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
IHC image of ab1107 staining in human breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1107, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Novel treatment option for MUC16-positive malignancies with the targeted - Novel treatment option for MUC16-positive malignancies with the targeted
Garg G, Gibbs J, Belt B, Powell MA, Mutch DG, Goedegebuure P, Collins L, Piwnica-Worms D, Hawkins WG, Spitzer D. BMC Cancer. 2014 Jan 21;14:35.
The use of ovarian cancer cells from patients undergoing surgery to generate - The use of ovarian cancer cells from patients undergoing surgery to generate
O Donnell RL, McCormick A, Mukhopadhyay A, Woodhouse LC, Moat M, Grundy A, Dixon M, Kaufman A, Soohoo S, Elattar A, Curtin NJ, Edmondson RJ. PLoS One. 2014 Mar 6;9(6):e90604.
New construction of an animal model for the orthotopic transplantation of an - New construction of an animal model for the orthotopic transplantation of an
Zhang H, Gao X, Yang Y, Wang W, Liu J, Liang Y, Wu H, Qin J, Pan K, Wang Y, Shi J, Ma Y. J Ovarian Res. 2014 Jun 11;7:64.
Mucin 16 is a functional selectin ligand on pancreatic cancer cells. - Mucin 16 is a functional selectin ligand on pancreatic cancer cells.
Chen SH, Dallas MR, Balzer EM, Konstantopoulos K. FASEB J. 2012 Mar;26(3):1349-59.
Overexpression of the cell adhesion molecules DDR1, Claudin 3, and Ep-CAM in - Overexpression of the cell adhesion molecules DDR1, Claudin 3, and Ep-CAM in
Heinzelmann-Schwarz VA, Gardiner-Garden M, Henshall SM, Scurry J, Scolyer RA, Davies MJ, Heinzelmann M, Kalish LH, Bali A, Kench JG, Edwards LS, Vanden Bergh PM, Hacker NF, Sutherland RL, O'Brien PM. Clin Cancer Res. 2004 Jul 1;10(13):4427-36.