Anti-MUM1 antibody [EP5699]

• Supplier: Abcam
• Catalog: ab133590
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP5699
• Applications: WB IHC-P IP FC ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to residues in Human MUM1 (UniProt ID: Q15306)
• Description: Rabbit Monoclonal
• Gene: IRF4
• Conjugate: Unconjugated

Product images

Overlay histogram showing Ramos cells stained with ab133590 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133590, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-MUM1 antibody [EP5699] (ab133590) at 1/10000 dilutionLane 1 : Namalwa cell lysateLane 2 : HuT78 cell lysateLane 3 : IM9 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling MUM1, using ab133590 at a dilution of 1/250.

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling MUM1, using ab133590 at a dilution of 1/250.