IRF4

Gene Symbol IRF4
Entrez Gene 3662
Alt Symbol LSIRF, MUM1, NF-EM5, SHEP8
Species Human
Gene Type protein-coding
Description interferon regulatory factor 4
Other Description lymphocyte-specific interferon regulatory factor|multiple myeloma oncogene 1
Swissprots Q15306 Q99660 Q5VUI7
Accessions EAW55056 EAW55057 EAW55058 EAW55059 EAW55060 Q15306 AK130762 AK310149 AW197089 BC015752 AAH15752 BG230623 BM551611 CR977713 D78261 BAA11335 DQ893472 ABM84398 DQ896302 ABM87301 U52682 AAC50779 U63738 AAB37258 XM_006715090 XP_006715153 NM_001195286 NP_001182215 NM_002460 NP_002451 NR_046000 NR_036585
Function Transcriptional activator. Binds to the interferon- stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity). {ECO:0000250|UniProtKB:Q64287}.
Subcellular Location Nucleus.
Tissue Specificity Lymphoid cells.

IRF-4 (D43H10) Rabbit mAb - 4299 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D43H10
Western blot analysis of extracts from various cell lines using IRF-4 (D43H10) Rabbit mAb.

IRF-4 (P173) Antibody - 4948 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from KARPAS-620 (human) and A20 (mouse) cells using IRF-4 Antibody #4964 and IRF-4 (P173) Antibody #4948.

IRF-4 Antibody - 4964 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Karpas-620 (human) and A20 (mouse) cell lines using IRF-4 Antibody #4964 and IRF-4 (P173) Antibody #4948.

IRF-4 (F-4) - sc-48338 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IRF-4 (F-4): sc-48338. Western blot analysis of IRF-4 expression in Ramos (A) and MM-142 (B) nuclear extracts.

IRF-4 (E-7) - sc-377383 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IRF-4 (E-7): sc-377383. Western blot analysis of IRF-4 expression in MM-142 whole cell lysate.

IRF-4 (H-140) - sc-28696 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
IRF-4 (H-140): sc-28696. Western blot analysis of IRF-4 expression in MM-142 (A) and Ramos (B) whole cell lysates.

IRF-4 (3E4) - sc-130921 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
IRF-4 (3E4): sc-130921. Western blot analysis of IRF-4 expression in non-transfected: sc-117752 (A) and human IRF-4 transfected: sc-176207 (B) 293T whole cell lysates.

IRF-4 (M-17) - sc-6059 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
IRF-4 (M-17): sc-6059. Western blot analysis of IRF-4 expression in MM-142 (A), HuT 78 (B) and Ramos (C) whole cell lysates.

IRF-4 (N-18) - sc-11450 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
IRF-4 (N-18): sc-11450. Western blot analysis of IRF-4 expression in non-transfected: sc-117752 (A) and human IRF-4 transfected: sc-176207 (B) 293T whole cell lysates.

IRF-4 (MUM1p) - sc-56713 from Santa Cruz Biotechnology

Prices $279.00
Sizes 250 µl supernatant
Host Mouse
Clonality Monoclonal
IRF-4 (MUM1p): sc-56713. Western blot analysis of IRF-4 expression in Ramos (A), NAMALWA (B), SR (C) and GA-10 (D) whole cell lysates.

Anti-MUM1 antibody - ab104803 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MUM1 antibody (ab104803) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

Anti-MUM1 antibody - ab66069 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
All lanes : Anti-MUM1 antibody (ab66069) at 1 µg/mlLane 1 : MUM1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysate. Lysates/proteins at 50 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-MUM1 antibody - ab101168 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MUM1 antibody - ab52713 from Abcam

Prices $359.00
Sizes 100 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-MUM1 antibody (ab52713) at 1/1000 dilutionLane 1 : ~20µg of a total protein extract from E coli with ~50ng to 100ng of a tagged fusion protein of an irrelevant antigen Lane 2 : ~20µg of a total protein extract from E coli with ~50ng to 500ng of the antigen (tagged antigen fusion protein)SecondaryRabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

Anti-MUM1 antibody - ab133790 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin fixed, paraffin-embedded Human thymus tissue labelling MUM1 with ab133790 at 5µg/ml. Primary incubation was followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-MUM1 antibody - ab133162 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-MUM1 antibody (ab133162) at 0.5 µg/ml (incubation time: 1 hour) + Jurkat cell lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-MUM1 antibody - ab183861 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MUM1 antibody (ab183861) at 1/1000 dilution + NCI-H929 whole cell lysate/extract at 30 µg

Anti-MUM1 antibody [2F2] - ab115765 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2F2
ab115765, at 5 µg/ml, staining MUM1 in formalin fixed, paraffin embedded Human neutrophils by Immunohiostochemistry.

Anti-MUM1 antibody [EP5699] - ab133590 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP5699
Overlay histogram showing Ramos cells stained with ab133590 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133590, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-MUM1 antibody [EPR5653] - ab124691 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5653
All lanes : Anti-MUM1 antibody [EPR5653] (ab124691) at 1/1000 dilutionLane 1 : Ramos cell lysateLane 2 : HuT 78 cell lysateLane 3 : IM 9 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-MUM1 antibody [EPR5654(2)] - ab133298 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5654(2)
All lanes : Anti-MUM1 antibody [EPR5654(2)] (ab133298) at 1/1000 dilutionLane 1 : IM-9 cell lysateLane 2 : HuT-78 cell lysateLysates/proteins at 10 µg per lane.

Anti-MUM1 antibody [MUM1p] - ab12501 from Abcam

Prices $382.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone MUM1p
IHC image of ab12501 staining in human Hodgkin's lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12501, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-IRF4 Antibody (aa116-165) - LS-C31075 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Jurkat Cell Lysate

Anti-IRF4 Antibody (aa130-451) - LS-C125790 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-IRF4 Antibody (N-Terminus) - LS-C125791 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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