All lanes : Anti-MUM1 antibody [EPR5653] (ab124691) at 1/1000 dilutionLane 1 : Ramos cell lysateLane 2 : HuT 78 cell lysateLane 3 : IM 9 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution
ab124691, at 1/100, staining MUM1 in paraffin-embedded Human colon tissue by Immunohistochemistry.
ab124691, at 1/100, staining MUM1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Immunohistochemical analysis of dog spleen tissue, staining MUM1 with ab124691.Tissue was fixed with formaldehyde and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a TRIS-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/50 in PBS plus 1X casein) for 1 hour 30 minutes at 37°C. An undiluted HRP-conjugated horse anti-rabbit polyclonal IgG was used as the secondary antibody. See Abreview
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD