Anti-Mus81 antibody [MTA30 2G10/3]

• Supplier: Abcam
• Catalog: ab14387
• Prices: $394.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: MTA30 2G10/3
• Applications: ICC/IF ICC/IF WB IP FC
• Species Reactivities: Human
• Antigen: Mus81 protein
• Description: Mouse Monoclonal
• Gene: MUS81
• Conjugate: Unconjugated

Product images

Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution + HeLa cell lysate

Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

Substrate specificity of the MUS81-EME2 structure selective endonuclease. - Substrate specificity of the MUS81-EME2 structure selective endonuclease.

Pepe A, West SC. Nucleic Acids Res. 2014 Apr;42(6):3833-45.

Essential role for Cdk2 inhibitory phosphorylation during replication stress - Essential role for Cdk2 inhibitory phosphorylation during replication stress

Hughes BT, Sidorova J, Swanger J, Monnat RJ Jr, Clurman BE. Proc Natl Acad Sci U S A. 2013 May 28;110(22):8954-9. doi:

ERCC1 and MUS81-EME1 promote sister chromatid separation by processing late - ERCC1 and MUS81-EME1 promote sister chromatid separation by processing late

Naim V, Wilhelm T, Debatisse M, Rosselli F. Nat Cell Biol. 2013 Aug;15(8):1008-15.

MUS81 promotes common fragile site expression. - MUS81 promotes common fragile site expression.

Ying S, Minocherhomji S, Chan KL, Palmai-Pallag T, Chu WK, Wass T, Mankouri HW, Liu Y, Hickson ID. Nat Cell Biol. 2013 Aug;15(8):1001-7.

Perturbed replication induced genome wide or at common fragile sites is - Perturbed replication induced genome wide or at common fragile sites is

Murfuni I, De Santis A, Federico M, Bignami M, Pichierri P, Franchitto A. Carcinogenesis. 2012 Sep;33(9):1655-63.

CtIP is required to initiate replication-dependent interstrand crosslink repair. - CtIP is required to initiate replication-dependent interstrand crosslink repair.

Duquette ML, Zhu Q, Taylor ER, Tsay AJ, Shi LZ, Berns MW, McGowan CH. PLoS Genet. 2012;8(11):e1003050.

SLX4, a coordinator of structure-specific endonucleases, is mutated in a new - SLX4, a coordinator of structure-specific endonucleases, is mutated in a new

Stoepker C, Hain K, Schuster B, Hilhorst-Hofstee Y, Rooimans MA, Steltenpool J, Oostra AB, Eirich K, Korthof ET, Nieuwint AW, Jaspers NG, Bettecken T, Joenje H, Schindler D, Rouse J, de Winter JP. Nat Genet. 2011 Feb;43(2):138-41.

Wee1 controls genomic stability during replication by regulating the Mus81-Eme1 - Wee1 controls genomic stability during replication by regulating the Mus81-Eme1

Dominguez-Kelly R, Martin Y, Koundrioukoff S, Tanenbaum ME, Smits VA, Medema RH, Debatisse M, Freire R. J Cell Biol. 2011 Aug 22;194(4):567-79.

Mus81-mediated DNA cleavage resolves replication forks stalled by topoisomerase - Mus81-mediated DNA cleavage resolves replication forks stalled by topoisomerase

Regairaz M, Zhang YW, Fu H, Agama KK, Tata N, Agrawal S, Aladjem MI, Pommier Y. J Cell Biol. 2011 Nov 28;195(5):739-49.

Mechanism of Holliday junction resolution by the human GEN1 protein. - Mechanism of Holliday junction resolution by the human GEN1 protein.

Rass U, Compton SA, Matos J, Singleton MR, Ip SC, Blanco MG, Griffith JD, West SC. Genes Dev. 2010 Jul 15;24(14):1559-69.