Anti-Musashi 1 / Msi1 antibody

• Supplier: Abcam
• Catalog: ab21628
• Prices: $391.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Musashi 1/ Msi1
• Blocking Peptide: Human Musashi 1 / Msi1 peptide
• Description: Rabbit Polyclonal
• Gene: MSI1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Musashi 1 / Msi1 antibody (ab21628) at 1 µg/mlLane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 2 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)Lane 3 : E14 Mouse Embryo Brain Tissue LysateLane 4 : E10 Mouse Embryo Brain Tissue Lysate Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/mlLane 6 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/mlLane 7 : E14 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/mlLane 8 : E10 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

IHC-FoFr image of Musashi/Msi1 staining on rat SVZ sections using ab21628 (1:300). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.See Abreview

ab21628 at 1ug/ml staining human eye (limbal region) by ICC/IF. The cells were paraformaldehyde fixed and blocked with 2% BSA prior to incubation with antibody for 1 hour. A FITC conjugated sheep polyclonal antibody was used as the secondary.See Abreview

ICC/IF image of ab21628 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21628, 10µg/ml) overnight at +4°C. The secondary antibody (green) was a96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 10µg/ml.

Product References

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