MSI1

Gene Symbol MSI1
Entrez Gene 4440
Alt Symbol -
Species Human
Gene Type protein-coding
Description musashi RNA-binding protein 1
Other Description RNA-binding protein Musashi homolog 1|musashi-1|musashi1
Swissprots Q96PU1 Q96PU2 Q96PU0 O43347 Q96PU3
Accessions AAB95636 CBF67590 CBU92464 EAW98185 EAW98186 EAW98187 O43347 AB012851 BAA33962 AB072590 BAB69767 AB072591 BAB69768 AB072592 BAB69769 AB073212 BAB70469 AI583233 AK023262 BAG51178 BC140380 AAI40381 BC146463 AAI46464 BX393884 XM_006719403 XP_006719466 XM_006719404 XP_006719467 XM_011538361 XP_011536663 XM_011538362 XP_011536664 XM_011538363 XP_011536665 XM_011538364 XP_011536666 XM_011538365 XP_011536667 XM_011538366 XP_011536668 XM_011538367 XP_011536669 XM_011538368 XP_011536670 XM_011538369 XP_011536671 XM_011538370 XP_011536672 XM_011538371 XP_011536673 NM_002442 NP_002433
Function RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'- [GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
Tissue Specificity Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. {ECO:0000269|PubMed:12054577, ECO:0000269|PubMed:9790759}.
Top Pathways mRNA surveillance pathway

Musashi-1 (D46A8) Rabbit mAb - 5663 from Cell Signaling Technology

Prices $139.00, $269.00
Sizes 40 µl (4 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D46A8
Western blot analysis of extracts from neonatal mouse brain and fetal rat brain using Musashi-1 (D46A8) XP ® Rabbit mAb.

Musashi Antibody - 2154 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from IEC-6 and LS174T cells, using Musashi Antibody.

Msi1 (H-45) - sc-98845 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Msi1 (H-45): sc-98845. Western blot analysis of Msi1 expression in IMR-32 whole cell lysate.

Msi1 (N-14) - sc-26278 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Msi1 (69-Q) - sc-135721 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Msi1 (69-Q): sc-135721. Western blot analysis of Msi1 expression in IMR-32 whole cell lysate.

Anti-Musashi 1 / Msi1 antibody - ab21628 from Abcam

Prices $391.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Musashi 1 / Msi1 antibody (ab21628) at 1 µg/mlLane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 2 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)Lane 3 : E14 Mouse Embryo Brain Tissue LysateLane 4 : E10 Mouse Embryo Brain Tissue Lysate Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/mlLane 6 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/mlLane 7 : E14 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/mlLane 8 : E10 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Anti-Musashi 1 / Msi1 antibody - ab60600 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-Musashi 1 / Msi1 antibody (ab60600) + full length recombinant protein with tag at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilutiondeveloped using the ECL technique

Anti-Musashi 1 / Msi1 antibody [3F8-E6-B9] - ab150762 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F8-E6-B9
All lanes : Anti-Musashi 1 / Msi1 antibody [3F8-E6-B9] (ab150762) at 1/1000 dilutionLane 1 : U-87 MG cell lysatesLane 2 : C6 cell lysatesLane 3 : IMR32 cell lysatesLane 4 : PC12 cell lysatesLane 5 : Mouse brain lysates

Anti-Musashi 1 / Msi1 antibody [EP1302] - ab52865 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1302
Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution + SH-SY-5Y cell lysate at 10 µg/mlSecondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-Musashi 1 / Msi1 antibody [EP1302] (Alexa Fluor® 488) - ab199781 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1302
ab199781 staining Musashi 1 / Msi1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199781 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Musashi 1 / Msi1 antibody [EP1302] (Alexa Fluor® 647) - ab199838 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1302
ab199838 staining Musashi 1 / Msi1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199838 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5 min).

Anti-Musashi 1 / Msi1 antibody [EP1302] (HRP) - ab199556 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1302
Anti-Musashi 1 / Msi1 antibody [EP1302] (HRP) (ab199556) at 1/5000 dilution + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Musashi 1 / Msi1 antibody [Y2709] - ab129819 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone Y2709
All lanes : Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819) at 5 µg/mlLane 1 : Brain (Human) Membrane Lysate - fetal normal tissue (ab29457)Lane 2 : E14 Rat Embryo Brain Tissue LysateLane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 4 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Musashi 1 / Msi1 antibody - N-terminal - ab170378 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Musashi 1 / Msi1 antibody - N-terminal (ab170378) at 1/100 dilution + CEM cell lysate at 35 µg

Anti-MSI1 / Musashi 1 Antibody (aa5-21) - LS-C25671 from LifeSpan Bioscience

Prices $475.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-MSI1 / Musashi 1 Antibody (clone 3E4) - LS-C104996 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E4

Anti-MSI1 / Musashi 1 Antibody (clone 2E9) - LS-C172558 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E9
IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MSI1 mouse monoclonal antibody.

Anti-MSI1 / Musashi 1 Antibody (clone 3G4) - LS-C337249 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3G4
IHC of paraffin-embedded Human Ovary tissue using anti-MSI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Anti-MSI1 / Musashi 1 Antibody (aa200-250) IHC-plus™ - LS-B7245 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MSI1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7245 concentration 10 ug/ml.

Anti-MSI1 / Musashi 1 Antibody (clone 2H9) - LS-C337218 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2H9
IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MSI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Anti-MSI1 / Musashi 1 Antibody (clone 1G8) - LS-C172522 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1G8
IHC of paraffin-embedded Human pancreas tissue using anti-MSI1 mouse monoclonal antibody.

Anti-MSI1 / Musashi 1 Antibody (clone 4C7) - LS-C104997 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4C7

Anti-MSI1 / Musashi 1 Antibody (clone 4A11) - LS-C104995 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4A11
Detection limit for recombinant GST tagged MSI1 is approximately 0.3 ng/ml as a capture antibody.

Anti-MSI1 / Musashi 1 Antibody (clone 1B11) - LS-C104994 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1B11
Immunoperoxidase of monoclonal antibody to MSI1 on formalin-fixed paraffin-embedded human kidney (antibody concentration 3 ug/ml).

Anti-MSI1 / Musashi 1 Antibody (aa1-362) - LS-C150010 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Musashi-1 in Human Intestine. Musashi-1 was detected in immersion fixed paraffin-embedded sections of human intestine using 1 ug/ml Human Musashi-1 Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in intestinal glands.
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