All lanes : Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819) at 5 µg/mlLane 1 : Brain (Human) Membrane Lysate - fetal normal tissue (ab29457)Lane 2 : E14 Rat Embryo Brain Tissue LysateLane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 4 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.